- Qingqingquan
- Shandong
- 20 days
- 300 units per month
Discover why AAALAC-certified labs use RO+UV+Ultrafiltration water systems for SPF animals. Learn how to achieve <0.05 EU/mL endotoxin & reduce microbial contamination by 99.9%
Ultrapure water solutions for laboratory animal breeding facilities
I. Laboratory Animal Drinking Water Standards Explained
1. Why do SPF animals need heat-free water?
• Reliability of experimental data: endotoxin (>0.25 EU/mL) may interfere with experimental results
• Animal Health Impact: Pathogenic Microorganisms (e.g. Pseudomonas) Cause Animal Disease
• Industry regulation: AAALAC/GLP requires animal drinking water with TOC <500ppb and resistivity ≥1MΩ-cm
2. Data Support:
• Research shows that the use of RO water can reduce the rate of intestinal microbial abnormalities in mice by 37% (Nature Methods, 2023).
II. Core technology: RO+UV+Ultrafiltration combined process
How to ensure that the water quality continuously meets the standard?
1. Reverse osmosis (RO):
• Remove 99% of ions, organic matter, resistivity ≥ 5MΩ-cm
2. UV sterilization (254nm):
• Inactivate microorganisms, reduce endotoxin activity
3. Ultra-filtration terminal processing:
• 0.1μm pore size retention of bacteria / pyrogens
Comparison of system configuration:
| Indicators | General RO System | Laboratory-specific System |
|---|---|---|
| Endotoxin Control | <0.5 EU/mL | <0.05 EU/mL |
| Microbial Compliance | 90% | 99.9% |
| Piping Material | PVC | 316L Stainless Steel |
III. Key Challenge: Avoiding Secondary Contamination
3 Key Points in Piping System Design
1. Circulation disinfection design:
• Regular flushing with ozone + hot water (80°C)
2. Dead space control:
• Pipe length/diameter ratio ≤ 3:1, flow rate > 1.5m/s
3. Material selection:
• Avoid PVC release plasticizer, recommended sanitary stainless steel
IV. Case: a GLP laboratory upgrade practice
From ordinary RO to AAALAC accreditation transformation
1. Original Issue:
• Drinking water endotoxin fluctuation (0.3-1.2 EU/mL)
• Animal diarrhea rate 8% (2x over standard)
2. Solution:
• Installation of integrated RO+UV+Ultrafiltration system
• Replacement of all stainless steel piping
3. Results:
✅ Endotoxin stabilized <0.03 EU/mL
✅ Animal health anomaly rate reduced to 1.2%
✅ Passed AAALAC annual audit
V. Equipment Selection Guide
SPF Facility Water System Essential Features
1. Real-time monitoring:
TOC/resistivity/endotoxin on-line detection
2. Expandability:
Modular design supports future expansion
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